General clinical trials included clinical blood analysis, urinalysis, biochemical blood analysis (total protein, glucose, creatinine, urea, sialic, sublimate, thymol samples, irubin, β – lipoproteins, cholesterol , amylase, calcium , phosphorus, alkaline phosphatase, transaminases, lactate dehydrogenase, fibrinogen, prothrombin).
Cytological examination (myelogram) was performed for all patients with myeloma to verify the diagnosis. Sternal puncture was prescribed to patients with CLL according to indications in most cases for the diagnosis of stage 0 of the disease (according to K. Rai classification, 1975). If during the initial diagnosis of CLL, an operative lymph node biopsy was performed, prints were prepared for cytological examination.
Intravital histological studies (surgical biopsy of the lymph node, trephine biopsy of the ilium) were performed by most patients with CLL to verify the diagnosis and differential diagnosis of CLL with mature lymphomas from B lymphocytes in the leukemia stage. A histological examination of the lymph node in patients with CLL also led to the diagnosis of Richter syndrome (transformation of CLL into a large cell lymphosarcoma). To verify the diagnosis of multiple focal MM and solitary myeloma, a histological study of material taken during surgical biopsy from a myeloma tumor or destruction was performed.
The function of external respiration was determined using the “Fukuda” apparatus (Japan) and included spirometry, computer analysis of the “flow-volume” loop. Criteria developed by N.V. Putovym and G.B. Fesedeev. The severity of ventilation disorders was assessed by a 3-point system: moderate (1 degree), significantly (2 degree) and sharply (3 degree) pronounced disorders.